Research

Zheng Z, Yin J, Cheng B, Huang W. Materials Selection for the Injection into Vaginal Wall for Treatment of Vaginal Atrophy. Aesthetic Plast Surg. 2021;45(3):1231-1241. Click to read<—<

Runels CE, Melnick H, DeBourbon E., A Pilot Study of the Effect of Localized Injections of Autologous Platelet Rich Plasma (PRP) for the Treatment of Female Sexual Dysfunction. J Women’s Health Care 2014, 3:4
—>>>Click here to read. The introduction explains the science of the O-Shot®Runels – 2014 – A Pilot Study of the Effect of Localized Injection

>>—-The Use of PRP in a Gynecology Setting (notice the extensive references at the end of this paper)<–

>>–Neto JB, O-Shot®: O-Shot: Platelet Rich Plasma in Intimate Female Treatment – oshot-platelets-rich-plasma-in-intimate-female-treatment-2167-0420-1000395

Chapter 15 of the following medical textbook includes a chapter (written by Dr. Runels) about the O-Shot® procedure with references to the research…

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration<–

Cheng-Yu, Long; Kun-Ling, Lin; Chin-Ru, Shen; Chin-Ru, Ker; Yi-Yin, Liu; et al. Scientific Reports (Nature Publisher Group); London Vol. 11, Iss. 1,  (2021). DOI:10.1038/s41598-020-80598-2

A pilot study: effectiveness of local injection of autologous platelet-rich plasma in treating women with stress urinary incontinence

Laser combined with the O-Shot procedure for urinary incontinence in women.

Matz, EL: Safety and feasibility of platelet-rich fibrin matrix injections for treatment of common urologic conditions Demonstrates O-Shot® methodology could be widely effective for stress incontinence in Women

—>>>>O-Shot® for interstitial cystitis<– 
We have found that inter-vesicular injections are not necessary–we see great results with our usual O-Shot® technique

O-Shot® for stress urinary incontinence in British Journal of Medicine<–

O-Shot® for stress urinary incontinence in Medical Hypothesis<–

23. Gafni-Kane, Adam MD; Sand, Peter K. MD. Foreign-Body Granuloma After Injection of Calcium Hydroxylapatite for Type III Stress Urinary Incontinence. Obstetrics & Gynecology: August 2011 – Volume 118 – Issue 2, Part 2 – pp 418-421doi:10.1097/AOG.0b013e3182161953 [showed a significant incidence of granuloma formation after injectin Radiesse like materials to treat urinary incontinence. Conclusion is that benefit is worth the risk].

24. Alijotas-Reig, Jaume MD, PhD. Foreign-Body Granuloma After Injection of Calcium Hydroxylapatite for Treating Urinary Incontinence. Obstetrics & Gynecology: November 2011-Volume 118- Issue 5-p 1181-1182 doi: 10.1097/AOG.0b-12e318235962d [Letter to editor challenging the idea that the benefit is worth the risk when using Radiesse like materials to treat stress incontinence–the point is that even with a risk of complications that require surgery, some gynecologist consider injecting around the urethera with Radiesse or Juvederm like material (i.e. the G-Shot™) to be an accepted practice. Yet with the PRP used in the O-Shot™ there seems to be ZERO risk of granuloma with even more benefit]

25. The American College of Obstetricians and Gynecologists,Practice_Bulletin_No__119__Female_Sexual.38
[Therapy available, hormones and psychotherapy–that’s it]

Hersant B, et al, Efficacy of injecting platelet concentrate combined with hyaluronic acid for the treatment of vulvovaginal atrophy in postmenopausal women with a history of breast cancer: a phase 2 pilot study. Menopause 2018, Vol 25, No. 10, pp. 1124-1130

**Rejuvenation Using Platelet-rich Plasma and Lipofilling for Vaginal Atrophy and Lichen Sclerosus (click to read)<–

Posey K, Runels C, In-Office Surgery and Use of Platelet Rich Plasma for Treatment of Vulvar Lichen Sclerosus to Alleviate Painful Sexual Intercourse, Journal of Lower Genital Tract Disease. 2017 Vol 21, #45. S14

Goldstein AT, King M, Runels C, Gloth M, Pfau R, Intradermal injection of autologous platelet-rich plasma for the treatment of vulvar lichen sclerosus. Journal of the American Academy of Dermatology, 2017, 76(1), 158-160 (click to read)

**Platelet-rich plasma (PRP) for the treatment of vulvar lichen sclerosus in a premenopausal woman: A case report (this one with dramatic photos)<<click to read<—.

** The use of PRP(platelet-rich plasma) in patients affected by genital lichen sclerosus: clinical analysis and results. <–click to read<==

**The first study with biopsies showing benefit from PRP for lichen sclerosus (using the O-Shot® techniques). The dermatopathologists were blinded to the before and after treatments and benefit was shown…
click to read<—(scroll to the next to last abstract)

**More research about lichen sclerosus treatment with PRP
click to read<–

**Combining PRP with surgery for clitoral-hood phimosis from lichen sclerosus using O-Shot® techniques…
Click to read<– (it’s on page S14)

Find provider who will consider treating lichen sclerosus with PRP (RESULTS WILL VARY)…
click<–(see the legend)

Women suffering with lichen sclerosus–Apply to participate in our on-going research
click <–

Fabria, B: Use of Platelet-rich Plasma for Vulvovaginal Autoimmune Conditions Like Lichen Sclerosus. Shows that O-Shot® techniques can work for lichen sclerosus

Michelle King MSc, Hillary Tolson, Charles Runels MD, Meghan Gloth MD, Richard Pfau MD, Andrew T Goldstein MD (lead investigator). Autologous Platelet Rich Plasma (PRP) Intradermal Injections for the Treatment of Vulvar Lichen Sclerosus
–>> click here & scroll to next to last abstract to read

Oakley Sh, et al, Clitoral size and location in relation to sexual function using pelvic MRI J Sex Med. 2014 Apr:11 (4):1013-1022

—>> Here’s more about the O-Spot (click) (not the G-Spot)

Hom DB, Linzie BM, Huang TC. The healing effects of autologous platelet gel on acute human skin wounds. Arch Facial Plast Surg. 2007;9(3):174-183.

Sclafani AP, Romo TR III, Ukrainsky G, et al. Modulation of wound response and soft tissue ingrowth in synthetic and allogeneic implants with platelet concentrate. Arch Facial Plast Surg. 2005;7(3):163-169.

O’Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lowerextremity ulcers. Wound Repair Regen. 2008;16(6):749-756.

Cervelli, Valerio MD; Lucarini, Lucilla MD.Use of Platelet-Rich Plasma and Hyaluronic Acid in the Loss of Substance with Bone Exposure. Advances in Skin & Wound Care: April 2011 – Volume 24 – Issue 4 – pp 176-181 [This one shows using the same techniques used in the Vampire Facelift (R) procedure to grow new skin WHERE THERE IS NO SKIN]

Redaelli, Alessio. Face and neck revitalization with Platelet-rich plasma (PRP): clinical-outcome in a series of 23 consecutively treated patients. Journal of Drugs in Dermatology – May 1, 2010

Azzena B, Mazzoleni F, Abatangelo G, Zavan B, Vindigni V. Autologous platelet rich [Using Regen prepared platelet-derived growth factors (a single-spin centrifuge) to rejuvenate the face & neck] plasma as an adipocyte in vivo delivery system: case report. Aesthetic Plast Surg. 2008;32(1):155-161.

Cervelli V, Gentile P, Grimaldi M. Regenerative surgery: use of fat grafting combined10with platelet-rich plasma for chronic lower-extremity ulcers. Aesthetic Plast Surg. 2009;33(3):340-345.

Cervelli V, Palla L, Pascali M, De Angelis B, Curcio BC, Gentile P. Autologous

platelet-rich plasma mixed with purified fat graft in aesthetic plastic surgery. Aesthetic Plast Surg. 2009;33(5):716-721.

Cervelli V, Gentile P. Use of cell fat mixed with platelet gel in progressive hemifacial atrophy. Aesthetic Plast Surg. 2009;33(1):22-27.

Cervelli V, Gentile P, Scioli MG, et al. Application of platelet-rich plasma in plastic surgery: clinical and in vitro evaluation. Tissue Eng Part C Methods. 2009; 15:1-9.

Sclafani AP. Platelet-rich fibrin matrix for improvement of deep nasolabial folds. J Cosmet Dermatol. 2010;9(1):66-71.

15. Sclafani AP. Applications of platelet-rich fibrin matrix in facial plastic surgery. Facial Plast Surg. 2009;25(4):270-276.

16. Sclafani AP. Safety, efficacy, and utility of platelet-rich fibrin matrix in facial plastic surgery [published online February 21, 2011]. Arch Facial Plast Surg. 2011;13(4):247-251.

17. Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ, Kusumoto K.Proliferation-promoting effect of platelet-rich plasma on human adiposederived stem cells and human dermal fibroblasts. Plast Reconstr Surg. 2008; 122(5):1352-1360.

18. Oh DS, Cheon YW, Jeon YR, Lew DH. Activated platelet-rich plasma improves fat graft survival in nude mice: a pilot study. Dermatol Surg. 2011;37(5):619-625.

19. Danielsen P, Jørgensen B, Karlsmark T, Jorgensen LN, Agren MS. Effect of topical autologous platelet-rich fibrin versus no intervention on epithelialization of donor sites and meshed split-thickness skin autografts: a randomized clinical trial. Plast Reconstr Surg. 2008;122(5):1431-1440.

20. Anitua E, Sa´nchez M, Zalduendo MM, et al. Fibroblastic response to treatment with different preparations rich in growth factors. Cell Prolif. 2009;42(2):162-170.

21. Carroll RJ, Arnoczky SP, Graham S, O’Connell SM. Characterization of Autologous Growth Factors in Cascade Platelet-Rich Fibrin Matrix (PRFM). Edison, NJ:Musculoskeletal Transplant Foundation; 2005. Publication No. 128-XM 307C5.

22. Anthony P. Sclafani, MD; Steven A. McCormick, MD. Induction of Dermal Collagenesis, Angiogenesis,and Adipogenesis in Human Skin by Injection of Platelet-Rich Fibrin Matrix. Arch Facial Plast Surg.Published online October 17, 2011. [Biopsy-proven new blood vessel growth and new collegen using platelet-derived growth factors (using a single-spin centrifuge)]